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A CdSe/ZnS quantum dot (QD) based electrochemical immunoassay of phosphorylated bovine serum albumin (BSA-OP) as a protein biomarker is presented. The QDs were used as labels for amplifying electrochemical signals and were conjugated with a secondary anti-phosphoserine antibody in a heterogeneous sandwich immunoassay. In this assay, the model phosphorylated protein BSA-OP was added to the primary BSA antibody coated polystyrene microwells, and then the QD labeled anti-phosphoserine antibody was added for completing immunorecognition. Finally, the bound QD was dissolved in an acid-dissolution step and was detected by electrochemical stripping analysis. The measured current responses were proportional to the concentration of BSA-OP. Under optimal conditions, the voltammetric response was linear over the range of 0.5-500 ng mL(-1) of BSA-OP, with a detection limit of 0.5 ng mL(-1). It also shows good reproducibility with a relative standard deviation of 8.6%. This QD-based electrochemical immunoassay offers great promise for simple and cost-effective analysis of protein biomarkers. (C) 2010 Elsevier B.V. All rights reserved. Addresses: [Wang, Jun; Lin, Chiann-Tso; Wu, Hong; Du, Dan; Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA; [Pinwattana, Kulwadee; Chailapakul, Orawon] Chulalongkorn Univ, Fac Sci, Dept Chem, Bangkok 10330, Thailand; [Chailapakul, Orawon] Chulalongkorn Univ, Ctr Excellence Petr Petrochem & Adv Mat, Bangkok 10330, Thailand Reprint Address: Lin, YH, Pacific NW Natl Lab, 902 Battele Blvd Richland, Richland, WA 99352 USA. E-mail Address: [email protected]; [email protected] Funding Acknowledgement: Funding Agency Grant Number Office of the Higher Education Commission, Thailand
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تاریخ انتشار 2010